IDENTIFIKASI GUGUS FUNGSI PADA MINYAK ATSIRI DAUN SINGKIL (Premna corymbosa) MENGGUNAKAN SPEKTROSKOPI FOURIER TRANSFORM INFRARED (FTIR)
DOI:
https://doi.org/10.24127/bioedukasi.v17i2.15664Abstract
Essential oils are complex mixtures of volatile secondary metabolites with significant biological activities, including antibacterial, antioxidant, and anti-inflammatory properties. Singkil leaves (Premna corymbosa) represent a potential natural source of essential oils containing various bioactive compounds. This study aimed to identify the functional groups present in the essential oil extracted from singkil leaves using Fourier Transform Infrared (FTIR) spectroscopy. The essential oil sample was analyzed using a Bruker OPUS 8.9.7 FTIR spectrometer within a wavenumber range of 400–4000 cm⁻¹. The FTIR spectrum exhibited four characteristic absorption bands: O–H stretching at 3287.79 cm⁻¹, an overtone/combination band at 2100.64 cm⁻¹, aromatic C=C stretching at 1635.78 cm⁻¹, and fingerprint region peaks at 461.99–413.34 cm⁻¹. These features indicate the presence of phenolics, flavonoids, furanoid sesquiterpenes, and amide alkaloids in the essential oil. Spectral library matching provided candidate structures with high similarity; however, these identifications remain putative and require confirmation with complementary analytical techniques. Overall, FTIR successfully revealed the functional group profile of P. corymbosa essential oil and can serve as a fundamental tool for its chemical characterization and quality assessment.
References
Agatonovic-kustrin, S., Ristivojevic, P., & Gegechkori, V. 2020. Essential Oil Quality and Purity Evaluation via FT-IR Spectroscopy and Pattern Recognition Techniques. Applied sciences, 10(20): 1-12
Arisandi, R., Pujiarti, R., Lukmandaru, G., & Mulyana, B. 2023. Chemical Constituents Of Melaleuca Leucadendron Linn . Leaf Essential Oils Quality Under Different Collecting. Indonesian Journal of Forestry Research, 10(2): 195–205.
Berlian, A. R. S., Elviantari, & Wibawa, I. P. A. H. 2023. Penyulingan Minyak Atsiri Dari Tumbuhan Kalistemon Kebun Raya “Eka Karya” Bali Dengan Menggunakan Metode Uap Air. BIOMARAS: Journal of Life Science and Technology, 1(1): 36–41.
Binders, A. B. 2020. Physico-Mechanical and Hygro-Thermal Properties of Compressed Earth Blocks Stabilized with Industrial and Agro By-Product Binders. Materials, 13(17): 1-17
De Sousa, D. P., de Assis Oliveira, F., Arcanjo, D. D. R., da Fonsêca, D. V., Duarte, A. B. S., de Oliveira Barbosa, C., Ong, T. P., & Brocksom, T. J. 2024. Essential Oils: Chemistry and Pharmacological Activities—Part II. Biomedicines, 12(6).
Dewi, C. E., Saleh, C., Pratiwi, D. R., & Magdaleni, A. R. 2024. Potensi aktivitas antioksidan ekstrak metanol daun singkil. Jurnal Atomik, 9(2):137–144.
Dika, F., Riswanto, O., Windarsih, A., Christin, D., & Putri, A. 2023. An Integrated Authentication Analysis of Citrus aurantium L . Essential Oil Based on FTIR Spectroscopy and Chemometrics with Tuning Parameters. Indonesian Journal Of Pharmacy, 34(2): 205–217.
Dillasamola, D., Aldi, Y., Fitria, N., Oktomalioputri, B., Lestari, U., & Multia, R. 2025. Isolation of Apigenin from Sungkai (Peronema canescens) Leaves and Its Immunomodulatory Effects : An In Vivo Study on Granzyme B , Interferon-γ , and Perforin Expression with Supporting In Silico Analysis. Research, 1–23.
Emu, M. R. K., Franciscus Sinung Pranata, & Yuliana Reni Swasti. 2022. Kualitas Virgin Coconut Oil (VCO) Dengan Penambahan Minyak Daun Salam (Syzygium polyanthum). Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 7: 236–243.
Gunarathne, R., Marikkar, N., Mendis, E., Yalegama, C., Jayasinghe, L., & Ulpathakumbura, S. 2022. Mid-IR Spectral Characterization and Chemometric Evaluation of Different Solvent Extracts of Coconut Testa Flour. Journal of Food Chemistry and Nanotechnology, 8(3): 69–75.
Husein, S. G., Firmansyah, A., & Yusuf1, F. F. 2020. Analisis Kemometrika Spektrum Fourier transformed infrared (FTIR). J. Sains dan Teknol. Farm. Indones, IX(2): 45–55.
Marfina, A., Cahyono, E., & Mursiti, S. 2019. Indonesian Journal of Chemical Science Sintesis Nanopartikel Emas dengan Bioreduktor Minyak Atsiri Kayu Manis (Cinnamomum burmannii ). Indonesian Journal of Chemical Science, 8(2): 126-132.
Nazri, M. Z., Nor, S., Abd, A., Norulfairuz, D., Zaidel, A., & Yahya, J. S. 2025. Fourier Transform Infrared ( Ftir ) Spectroscopy , Gas Chromatography And Chemometric Analysis For Halal. Journal Of Academia, 13(2): 209–223.
Noor, R., Maryani, M., Retnoaji, B., & Rahayuningsih, E. 2025. Visibility, Selectivity, Optimization, and Interaction of Natural Tannin Dye from Ceriops tagal (Perr.) C.B. Rob. Extract for Plant Tissue Staining. Journal of Multidisciplinary Applied Natural Science, 5(3), 913–933.
Pribadi, T., Zakiyyah, Z., Anisa, N., Wijayadi, I., & Masitah, M. 2025. Organoleptic Evaluation and Consumer Preferences for Singkil Tea and Kombucha Products (Premna corymbose Rottl . Et Willd ). Jurnal Pembelajaran Dan Biologi Nukleus, 11(2): 594-610
Pribadi, T., Zakiyyah, Z., Zahra, S., Ranesti, I., Hadi, C., & Pratama, M. I. 2024. Eksplorasi Potensi Daun Singkil (Premna corymbosa Rottl. et Willd) sebagai Teh Herbal: Studi Perbandingan Kandungan Bioaktif Ekstrak dan Rendaman Daun. AGROTEKNIKA, 7(4): 576–588.
Smith, B. D. 2023. Advances in Optical Sensors of N-acetyl-β-D-Hexosaminidase (N-acetyl-β-D-Glucosaminidase). Advances, 33(4): 544–554.
Supriningrum, R., Handayani, F., & Liya. 2017. Karakterisasi dan Skrining Fitokimia Daun Singkil (Premna corymbosa Rottl & Willd). Jurnal Kimia Mulawarman, 13(2): 89–96.
Taupik, M., Mu, A., Suryadi, A., Hiola, F., & Rannu, J. 2021. Karakterisasi Senyawa Minyak Atsiri Ekstrak Etil Asetat Bawang Putih (Allium sativa). Indonesian Journal of Pharmaceutical Education, 1(2): 127–135.
Taylan, O., Cebi, N., & Sagdic, O. 2021. Rapid Screening of Mentha spicata Essential Oil and L-Menthol in Mentha piperita Essential Oil by ATR-FTIR Spectroscopy. Foods, 10(2): 202.
Tyagi, A., Yadav, A. K., Yadav, A., Saini, L., Kumar, V., Jain, P., Mohammad, I., Ansari, M. J., El Enshasy, H. A., Abdel-Gawad, F. K., Obaid, S. Al, Siddiqui, S. A., & Malik, V. (2022). Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach. Journal of King Saud University - Science, 34(8): 102355.
Wang, X., Li, Y., Wang, X., Sandoval, D. M., He, Z., Sigen, A., Lara, I., & Wang, W. 2023. Guanidyl-Rich Poly ( β Amino Ester ) s for Universal Functional Cytosolic Protein Delivery and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas9 Ribonucleoprotein Based Gene Editing. Biomedicines, 12(6): 1185
Zakiyyah, Z., Pribadi, T., Bonita, T., Wijayadi, I., Awang, E. R., & Masitah. 2025. Kajian Rendemen dan Mutu Organoleptik Minyak Atsiri Daun Singkil (Premna Corymbosa Rottl . Et Willd) Sebagai Kandidat Aroma Terapi. Eduproxima, 7(4): 2593–2606.
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Copyright (c) 2026 Teguh Pribadi, Ingghrid Renesti Clarita Hadi, Cindy Agriningsih Haruna, Niky Aulia Putri, Imam Wijayadi, Eadvin Rosrinda Awang Sari, Masitah Masitah

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