PENGARUH BAHAN ORGANIK EKSTRAK PISANG PADA MEDIA VACIN AND WENT TERHADAP PERTUMBUHAN FASE EMBRIO Phalaenopsis amabilis

Egi Nuryadin, Charisma Cinta Choeronisa, Edi Hernawan

Abstract


Phalaenopsis amabilis is the most popular type of orchid because this orchid has a unique shape so many people are interested in having it. However, the presence of orchids in nature, especially in the Galunggung Mountain area of Tasikmalaya is increasingly scarce so it is necessary to cultivate one of them through in vitro tissue culture. The purpose of this study was to determine the effect of giving organic ingredients of banana extract combined with vacin and went on the growth of the embryonic phase of orchids. This research was conducted in September 2018 - July 2019 at the Tissue Culture Laboratory, Department of Biology Education, Siliwangi University. The research method used is true experimental with a Completely Randomized Design (CRD) arrangement of 3 replications. With the treatment of five different concentrations namely (P1) 0gr / L, (P2) 10 gr / L, (P3) 20 gr / L, (P4) 30 gr / L and (P5) 40 gr / L. The parameter of this study is the time the embryo appears at each growth phase. The data analysis technique used is the one way ANOVA test with ? = 0.05 and the LSD advanced test. The results showed that the administration of banana extract organic material with five different concentrations had a significant effect, but at the concentration (P3) 20 gr / L was the best concentration which gave the most significant influence on the growth of orchid embryos in all embryonic phase growth times.

Kata kunci: Ekstrak pisang, Vacin and Went, Pertumbuhan, Phalaenopsis amabilis


References


Arditti, J., & Ernst, R. 1993. Micropropagation of Orchids. John Wiley and sons, New

York. Diakses 12 Januari 2019.

Djajanegara. 2010. Pemanfaatan Limbah Buah Pisang dan Air Kelapa Sebagai Bahan Media Kultur Jaringan Anggrek Bulan (Phalaenopsis amabilis) Tipe 229 .J.Tek. Ling. Vol.11 No.3 Hal. 373-380. Diakses 2 Januari 2019.

Djaafarer, R. 2003. Phalaenopsis spesies: jenis dan potensi untuk silangan. Jakarta: Penebar Swadaya.

Dwiyani, R. 2013. Perkecambahan Biji dan Pertumbuhan Protokorm Anggrek dari Buah dengan Umur yang Berbeda pada Media Kultur yang Diperkaya dengan Ekstrak Tomat. Bali. Jurnal Hortikultura

Indonesia 4(2):90-93. Diakses 12 Desember 2018.

Gunawan, L. W. 2003. Budi Daya Anggrek. Jakarta: Penebar Swadaya.

Hardjo, P. H. 2018. Kultur jaringan Anggrek. Surabaya: Graha Ilmu.

Kurniati, L. 2006. Pengaruh Konsentrasi Zat Pengatur Tumbuh Naa Dan Bap Terhadap Pertumbuhan Biji Dendrobium Capra J.J. Smith Secara In Vitro. Tugas Akhir, Diakses 5 Desember 2018.

Nuryadin, E. 2018. Perkecambahan Biji Dan Pengaruh Interaksi Zat Pengatur Tumbuh Terhadap Waktu Pertumbuhan Tanaman Kantong Semar (Nepenthes Adrianii) Secara In-Vitro Makalah disajikan dalam Seminar Nasional Biologi Tropika, Universitas Gadjah Mada, Yogyakarta, 28 Juli 2018.

Paramartha. 2012. Pengaruh Penambahan Kombinasi Konsentrasi ZPT NAA dan BAP terhadap Pertumbuhan dan Perkembangan Biji Dendrobium

Taurulinum J.J Smith Secara In Vitro: Jurnal Sains dan Seni ITS Vol. 1, No. 1, (Sept. 2012) ISSN: 2301-928X Diakses 17 November 2018.

Untari, R. dan Puspitaningtyas. 2006. Pengaruh bahan organik dan NAA tehadap pertumbuhan anggrek hitam (Coelogyne pandurata Lindl) dalam kultur In Vitro: jurnal Biodiversitas 7(3): 344-348. Diakses 9 November 2018.

Utami, E. S. W., Harianto, S., & Y.S.W. Manuhara. 2016. Pengaruh Pemberian Ekstrak Pisang pada Media VW terhadap Induksi Akar dan Pertumbuhan Tunas Dendrobium lasianthera J.J. Sm. Jurnal Agrotop. 6 (1):35-42 (2016). ISSN 2088-155X. Diakses 29 November 2018.




DOI: http://dx.doi.org/10.24127/bioedukasi.v11i1.2818

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